In vivo efficacy

Model features:

✔ Easy to handle, high tumorigenesis

✔ Low cost, more cost-effective

✔ Short experimental time, suitable for early screening

A. Tumor growth curve and body weight for Pan02 xenograft model

B. Test antibody inhibit MC38 growth in vivo

Model features:

✔ Immunodeficient mouse, efficacy focus on tumor itself

✔ Human cancer cell line

✔ Flexible, easy to handle, widely used

A. In vivo LC1sq, NCI-H2291, NCH-1581 NSCLC cancer cell line growth curves

Model features:

✔ Humanized Immune system in immunodeficient mouse, TME more similar to human

✔ Humanization can be conducted by Human PBMC or HSC

✔ Hard to handle

A. A549 in vivo growth curves (Human PBMC humanized NSG mice)

Model Features:

✔ Immunocompetent system

✔ Suitable for No-species-cross-reactivity antibodies

✔ Target humanized mice and tumor cell lines should be prepared in advance

A. In vivo anti-tumor activity of Keytruda and Opdivo in hPD1 mice

Orthotropic and metastasis tumor model can better manifest human tumor environment, keep original biological characteristics, and more relevant with clinical. Genscript Probio can provide a variety of orthotropic or metastasis tumor models to meet different needs from customers.

Models	Model Features	Tumor samples
Orthotropic tumor	Orthotropic growth High metastasis rate High clinical relevance Hard to handle, long experimental time	Lung Orthotropic A549 tumor
Metastasis tumor	More suitable for metastasis research Along with Orthotropic model Hard to handle, long experimental time	Lung Metastasis A549 tumor