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A cell-based reporter assay earns credits throughout the discovery through its reliable output, high throughput and cost-effectiveness. Compared to primary cells, it minimizes operations, variations and batch inconsistencies, all of which fosters the process of large-scale implementation of cell-based assays for lead evaluation, bioactivity analysis and further lot-release.
To promote the future of biologics, GenScript ProBio, guaranteed by professionals with comprehensive experience, offers a full package covering from design, cell line generation, assay development, optimization to qualification, all serving to fulfill your needs.
Contact us for more information about our Reporter Gene Assay Cell Line development services.
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The response window is an important indicator for reporter gene assay cell line . Rich experience in RE sequence optimization assists us to develop a customized assay with high sensitivity.
GenScript ProBio will scrutinize the key factors such as cell seeding density, serum concentration, and incubation conditions to achieve an optimal assay.
Two engineered cell lines - PD-L1/CD155 aAPC/CHO-K1 Cell and PD-1/TIGIT Effector Cell are used in this bioassay. The interaction between TIGIT and CD155, and PD-1 and PD-L1 inhibits TCR-mediated activation. When disrupting the interactions of both pairs, TCR activation will be restored indicated by the luminescence signal.
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